About RevGenUK
RevGenUK is a technology platform for reverse genetics in plants, subsidised by the BBSRC. It combines three plant species and two reverse genetic technologies onto a single technology platform. Two of the species – Lotus japonicus and Medicago truncatula - have been adopted as models for legume genomics by the community and have been used by the John Innes Centre to develop TILLING and de-TILLING technologies. TILLING in Lotus japonicus has been available for the last three years and more recently in Medicago truncatula. Currently we have assembled and are TILLING in populations in Lotus, Medicago and Brassica rapa, and have generated a population of fast-neutron mutagenised plants for Medicago.
Detection of mutations is usually carried out using gel-based systems (which are inefficient), but the John Innes Genome Laboratory has developed a highly efficient system that uses capillary sequencers (ABI3730 DNA). This will be used as our single technology platform for all three mutation detection programs in our target species. Data will be collated into an open source web-based database. In this way, under the umbrella of the single technology platform, we will create a unique resource for mutation discovery and functional genomics for the benefit of research community.
The platform, that will build on previous BBSRC investment in genomic resources, is designed to be capable of expansion as additional resources become available (e.g. Brachypodium), and will become self-sustaining in the future. We are open to collaborations on all aspects of our service, so if you are thinking of setting up TILLING in your own species, or just considering that you may want to do some TILLING on ours, get in touch with us!
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RevGenUK, John Innes Centre |
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